Composite

Part:BBa_I715037:Design

Designed by: Andrew Martens   Group: iGEM07_Davidson_Missouri_W   (2007-07-29)


HPP-B1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1856
    Illegal AgeI site found at 1968
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 727
    Illegal BsaI.rc site found at 1507


Design Notes

Alternatively, we may try building it in fewer steps doing a triple ligation.


Source

Combine parts BBa_S03763 and BBa_S03764.

References